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KMID : 0378020070500010011
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New Medical Journal
2007 Volume.50 No. 1 p.11 ~ p.17
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Isolation and Culture of Adult Stem Cells in Human Vestibular Organs - Preliminary Study -
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Yoo Young-Hwa
Seo Jae-Hyun
Hong Myoung-Wha
Park Shi-Nae
Chang Ki-Hong
Yeo Sang-Won
Park Kyoung-Ho
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Abstract
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Background and Objectives : It is known that damaged vestibular organs can generate new hair cells, albeit in limited numbers. In human, there is no report on human somatic stem cell in vestibular organ. We try to show that human vestibular sensory epithelium contain cells that display characteristic features of stem cells.
Materials & Method : Sensory epithelia of utricle, saccule and 3 semicircular canals were obtained from the patient with acoustic neuroma. The cells were cultured in a medium with epidermal growth factor (EGF) and fibroblast growth factor (FGF). Proliferating cells were checked with stem cell markers, bromodeoxyuridine (BrdU) and nestin.
Results: We observed that some of the cultured cells from 3 vestibular organs were stained with both stem cell markers, BrdU and nestin. But we can not subculture and differentiate these cells into organ specific differentiated cells, hair cells and supporting cells.
Conclusion: The sensory epithelium of human vestibular organs may have stem cells. But further study is needed to check differentiating into organ specific cells.
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KEYWORD
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Human vestibular organs, Stem cell
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